Codsiga ultrafiltration ee kala-soocidda borotiinka iyo nadiifinta

Tignoolajiyada Ultrafiltration waa tignoolajiyad cusub oo tayo sare leh oo kala saarista.Waxay leedahay sifooyinka habka fudud, faa'iido dhaqaale oo sarreeya, isbeddel weji ah ma jiro, isugeyn ballaaran oo kala soocida, badbaadinta tamarta, waxtarka sare leh, ma jirto wasakh sare, hawlgal joogto ah heerkulka qolka iyo wixii la mid ah.Maanta, maareeye Yang oo ka socda Beijing ayaa wax ka waydiiyay qalabkayaga ultrafiltration ee borotiinka sifaynta oo si faahfaahsan ula xidhiidhay tignoolajiyadayada.Hadda, tifaftiraha kooxda Shandong Bona ayaa soo bandhigi doona codsiga ultrafiltration ee kala-soocidda borotiinka iyo nadiifinta.

1. Macnaynta borotiinka, khamriga-saarista iyo xoogga saarista
Codsiyada ugu muhiimsan ee ultrafiltration ee nadiifinta borotiinka waa nadiifinta iyo xoojinta.Habka Ultrafiltration in desalination iyo fiirsashada waxaa lagu gartaa mugga Dufcaddii weyn, waqtiga hawlgalka gaaban iyo waxtarka sare ee soo kabashada borotiinka.Habka soo jireenka ah ee chromatography ka saarista steric si looga saaro walxaha kala duwan ee borotiinka ayaa lagu beddelay tignoolajiyada casriga ah ee ultrafiltration, taas oo noqotay tignoolajiyada ugu weyn ee nadiifinta borotiinka, isku-duubnida iyo feejignaanta maanta.Sanadihii la soo dhaafay, tignoolajiyada ultrafiltration ayaa si weyn loogu isticmaalay nadiifinta iyo dib u soo kabashada borotiinada qiimaha nafaqada sare leh ee ku jira farmaajo whey iyo soybean whey.Lactose iyo cusbada iyo qaybaha kale ee borotiinka, iyo sidoo kale baahiyaha dhabta ah ee si guul leh u dhamaystiraya nadiifinta, khamriga iyo xoojinta borotiinka.Isticmaalka tignoolajiyada ultrafiltration waxay sidoo kale xoogga saari kartaa serospecies immunoglobulins si loo daboolo baahida dhabta ah ee soosaarka borotiinka.

2. Kala qaybsanaanta borotiinka
Jajabka borotiinka waxaa loola jeedaa habka loo kala saaro qayb kasta oo ka mid ah qayb ka mid ah borotiinka qayb ahaan iyadoo loo eegayo kala duwanaanshaha guryaha iyo kiimikada (sida miisaanka molecular qaraabo, barta isoelectric, hydrophobicity, iwm) ee qayb kasta oo borotiin ah oo ku jira dareeraha quudinta.chromatography jel waa mid ka mid ah hababka ugu badan ee loo isticmaalo kala qaybinta macromolecules noolaha (gaar ahaan borotiinada).Marka la barbar dhigo chromatography dhaqameed, tignoolajiyada kala soocida ultrafiltration waxay leedahay rajo wanaagsan oo ku saabsan codsiga qaybinta iyo wax soo saarka warshadaha borotiinnada iyo enzymes-ka oo leh qiime dhaqaale oo muhiim ah sababtoo ah qiimaha hoose iyo xoojinta fudud.Ukun cad ayaa ah alaabta ceeriin ee ugu raqiisan si loo helo lysozyme iyo ovalbumin.Dhawaan, ultrafiltration waxaa badanaa loo isticmaalaa in lagu kala saaro ovalbumin iyo lysozyme iyo ukunta cad.

3. Ka saarida Endotoxin
Ka saarida Endotoxin waa mid ka mid ah noocyada codsiga ugu weyn ee tignoolajiyada ultrafiltration ee nadiifinta borotiinka.Habka wax soo saarka ee endotoxin waa mid aad u adag.Habka codsiga wax ku oolka ah, sababtoo ah borotiinka daawada ee uu soo saaro nidaamka prokaryotic waa sahlan tahay in lagu qaso endotoxin ay soo saarto jebinta derbiga unugyada bakteeriyada, iyo endotoxin, oo sidoo kale loo yaqaano pyrogen, waa nooc ka mid ah lipopolysaccharide.Ka dib marka la galo jidhka bini'aadamka, waxay keeni kartaa qandho, khalkhalka microcirculation, shoogga endotoxic iyo calaamado kale.Si loo ilaaliyo caafimaadka aadanaha, waxaa lagama maarmaan ah in si buuxda loo isticmaalo tignoolajiyada ultrafiltration si meesha looga saaro endotoxins.

Inkasta oo tignoolajiyada ultrafiltration si weyn loogu isticmaalo kala-soocidda iyo nadiifinta borotiinka, waxay sidoo kale leedahay xaddidaadyo gaar ah.Haddii miisaanka molecular ee labada alaab ee la kala saarayo uu ka yar yahay 5 jeer, laguma kala saari karo ultrafiltration.Haddii miisaanka unugyadu uu ka yar yahay 3kD, laguma saari karo ultrafiltration, sababtoo ah ultrafiltration waxaa badanaa lagu sameeyaa miisaanka ugu yar ee xuubka 1000 NWML.

Iyadoo horumarinta joogtada ah ee warshadaha bioengineering, shuruudaha sare ayaa loo soo bandhigay kala-soocidda hoose iyo tignoolajiyada nadiifinta.Hababka soo jireenka ah ee fiirsashada faakuumka, soo saarista dareeraha, sifaynta, centrifugation, roobabka iyo saarista pyrogen ma aha kuwo la heli karo si loo daboolo baahiyaha wax soo saarka mar dambe.Tiknoolajiyada Ultrafiltration waxay ku xidhan tahay in aad iyo aad loo isticmaalo sababtoo ah faa'iidooyinka ay ku leedahay kala-soocidda borotiinka.